Related Experiment Video
Updated: Jun 22, 2026
03:53
Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
Published on: November 10, 2023
Development of a multiplex real-time PCR for detection and differentiation of malignant catarrhal fever viruses in
Cristina W Cunha1, Lisa Otto, Naomi S Taus
1USDA-ARS, Washington State University, Pullman, 99164-6630, USA. cwcunha@vetmed.wsu.edu
Journal of Clinical Microbiology
|June 5, 2009
Abstract:
A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).
