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Related Experiment Video

Updated: Jun 22, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Single particle multichannel bio-aerosol fluorescence sensor.

P Kaye, W R Stanley, E Hirst

    Optics Express
    |June 5, 2009
    PubMed
    Summary
    This summary is machine-generated.

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    A new low-cost aerosol fluorescence sensor detects bio-aerosols for wide-area monitoring. This device identifies particles by their unique fluorescence signatures, aiding in environmental and biological threat detection.

    Area of Science:

    • Environmental Science
    • Biotechnology
    • Sensor Technology

    Background:

    • Bio-aerosol detection is crucial for public health and security.
    • Existing methods for wide-area bio-aerosol monitoring can be costly and complex.
    • There is a need for robust, low-cost sensors for unattended deployment.

    Purpose of the Study:

    • To develop and present a prototype low-cost, multi-channel aerosol fluorescence sensor.
    • To enable unattended deployment in medium to large area bio-aerosol detection networks.
    • To characterize the sensor's performance for detecting airborne particles.

    Main Methods:

    • A continuous-wave diode laser (660nm) detects and sizes particles (~1µm).
    • UV pulses (~280nm and ~370nm) excite bio-fluorophores like tryptophan and NADH.

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    Last Updated: Jun 22, 2026

    Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
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    Published on: November 2, 2009

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
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    Published on: May 5, 2016

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  • Fluorescence is measured across two bands for each excitation wavelength.

    • Main Results:

    • The sensor detects and sizes individual airborne particles.
    • It differentiates particles based on fluorescence signatures.
    • Preliminary data shows sensitivity to E.coli, BG spores, and non-biological aerosols.
    • Current measurement rates reach ~125 particles/s, with plans to increase to ~500 particles/s.

    Conclusions:

    • The prototype sensor offers a low-cost solution for bio-aerosol detection.
    • Its design is suitable for unattended, wide-area monitoring networks.
    • Further development aims to enhance measurement rates and broaden applications.