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Related Experiment Video

Updated: Jun 22, 2026

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Parallel single-cell light-induced electroporation and dielectrophoretic manipulation.

Justin K Valley1, Steven Neale, Hsan-Yin Hsu

  • 1Berkeley Sensor and Actuator Center, Department of Electrical Engineering and Computer Science, University of California Berkeley, Berkeley, CA 94720, USA. valleyj@eecs.berkeley.edu

Lab on a Chip
|June 5, 2009
PubMed
Summary

This study introduces patterned light for parallel single-cell electroporation, enhancing throughput and selectivity. The novel method integrates with optoelectronic tweezers for cell manipulation and maintains cell viability.

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Last Updated: Jun 22, 2026

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

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Published on: July 11, 2008

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

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The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
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The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Electroporation is crucial for introducing molecules into cells but faces limitations in throughput and selectivity.
  • Conventional electroporation methods struggle with parallel processing of single cells.

Purpose of the Study:

  • To develop a novel technique for parallel, single-cell electroporation using patterned light.
  • To integrate this technique with optoelectronic tweezers for advanced cell manipulation.
  • To optimize treatment parameters for cell viability and molecule delivery.

Main Methods:

  • Utilizing patterned light to generate virtual electrodes for parallel electroporation.
  • Integrating optoelectronic tweezers for precise single-cell manipulation.
  • Employing fluorescent dyes and dielectrophoretic responses to confirm electroporation.
  • Using microfluidic channels to maintain cell viability post-treatment.

Main Results:

  • Demonstrated parallel, single-cell electroporation using light-induced virtual electrodes.
  • Confirmed successful cell manipulation and electroporation with integrated optoelectronic tweezers.
  • Showcased high cell viability after treatment within the microfluidic device.
  • Determined optimal field dosage for propidium iodide delivery in HeLa cells.

Conclusions:

  • Patterned light-induced virtual electrodes offer a novel, high-throughput, and selective method for parallel single-cell electroporation.
  • The integrated platform enables precise cell manipulation and efficient molecule delivery while preserving cell viability.
  • This technique advances cell-based assays and therapeutic applications requiring controlled genetic or molecular material delivery.