Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 22, 2026

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
13:50

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

Microfabricated curtains for controlled cell seeding in high throughput microfluidic systems.

Adrian T O'Neill1, Nancy A Monteiro-Riviere, Glenn M Walker

  • 1Joint Department of Biomedical Engineering at The University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695-7115, USA.

Lab on a Chip
|June 5, 2009
PubMed
Summary

A novel microfabricated cell curtain enhances cellular assays by extending culture times and enabling high-throughput screening. This PDMS wall technology improves cell viability and population dynamics in microfluidic devices.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Meta-Analysis and Machine Learning Prediction of Protein Corona Composition across Nanoparticle Systems in Biological Media.

ACS nano·2025
Same author

Predicting tissue distribution and tumor delivery of nanoparticles in mice using machine learning models.

Journal of controlled release : official journal of the Controlled Release Society·2024
Same author

Meta-Analysis of Nanoparticle Distribution in Tumors and Major Organs in Tumor-Bearing Mice.

ACS nano·2023
Same author

An artificial intelligence-assisted physiologically-based pharmacokinetic model to predict nanoparticle delivery to tumors in mice.

Journal of controlled release : official journal of the Controlled Release Society·2023
Same author

Current and future directions of drug delivery for the treatment of mental illnesses.

Advanced drug delivery reviews·2023
Same author

Interval delivery of 5HT<sub>2A</sub> agonists using multilayered polymer films.

Journal of biomedical materials research. Part A·2023

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Traditional microfluidic chambers limit cell culture duration.
  • High-throughput screening requires robust cell culture methods.

Purpose of the Study:

  • To introduce and characterize a microfabricated cell curtain for enhanced cellular assays.
  • To demonstrate the utility of cell curtains in high-throughput microfluidic devices.

Main Methods:

  • Fabrication of a poly(dimethylsiloxane) (PDMS) cell curtain within microchambers.
  • Culturing human epidermal keratinocyte (HEK) colonies with and without curtains.
  • Integration of cell curtains into a 96-chamber microfluidic device for high-throughput screening.
  • Assessing cell viability and population dynamics after ethanol exposure.

More Related Videos

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Related Experiment Videos

Last Updated: Jun 22, 2026

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
13:50

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Main Results:

  • Cell curtains extended observation times for HEK colonies by over 48 hours.
  • Microfluidic devices with cell curtains enabled high-throughput screening of ethanol dilutions (0-22%).
  • Cells exposed to ≤12% ethanol showed ≥85% viability after 24 hours, while higher concentrations drastically reduced viability.
  • Ethanol concentrations ≤6% promoted cell growth, 8% stunted growth, and higher concentrations caused population loss.

Conclusions:

  • Microfabricated cell curtains significantly improve cell culture duration and viability in microfluidic systems.
  • The cell curtain technology facilitates high-throughput screening of cellular responses to chemical exposures.
  • This innovation supports advanced cellular assays and drug discovery platforms.