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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Related Experiment Video

Updated: Jun 22, 2026

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
12:47

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Published on: November 3, 2014

Studying autoimmunity by in vivo RNA interference.

Stephan Kissler1

  • 1Rudolf Virchow Center/DFG Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|June 5, 2009
PubMed
Summary

Genetic variants influence autoimmunity. Lentiviral RNA interference (RNAi) in animal models offers a faster way to study gene function in autoimmune diseases, bypassing complex genetic engineering. This aids in understanding disease causes.

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Area of Science:

  • Immunology
  • Genetics
  • Molecular Biology

Background:

  • Autoimmune diseases are linked to multiple gene variants influencing susceptibility.
  • Understanding gene polymorphisms is crucial for elucidating disease etiology and pathogenesis.
  • Human genetic studies identify candidate genes, but functional validation requires animal models.

Purpose of the Study:

  • To present lentiviral transgenesis combined with RNA interference (RNAi) as a method for genetic manipulation in experimental autoimmunity models.
  • To highlight the advantages of this approach over traditional methods for studying gene function in autoimmunity.

Main Methods:

  • Utilized lentiviral transgenesis and RNA interference (RNAi).
  • This technique facilitates genetic manipulation in animal models of autoimmunity.
  • Bypassed the need for embryonic stem cell mutagenesis and extensive animal backcrossing.

Main Results:

  • Lentiviral RNAi provides a streamlined method for genetic modification in autoimmunity research.
  • The approach allows for efficient functional studies of gene variants associated with autoimmune diseases.
  • Detailed discussion of the technique's strengths and limitations is provided.

Conclusions:

  • Lentiviral RNAi is a powerful tool for advancing the study of autoimmune disease genetics.
  • This method accelerates functional genetic studies in relevant animal models.
  • It offers a valuable alternative to conventional transgenesis and knockout technologies for autoimmunity research.