Related Experiment Video
Updated: Jun 22, 2026
High Throughput Image-Based Phenotyping for Determining Morphological and Physiological Responses to Single and Combined Stresses in Potato
Published on: June 7, 2024
Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
Federico Gómez Galindo1, Petr Dejmek, Krister Lundgren
1Centro de Engenharia Biológica, Institute for Biotechnology and Bioengineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal. Federico.Gomez@food.lth.se
Abstract:
Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.
