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Updated: Jun 22, 2026
06:45
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
Published on: February 28, 2019
Single-molecule mechanical unfolding of amyloidogenic beta2-microglobulin: the force-spectroscopy approach
1National Nanotechnology Laboratory of INFM-CNR, Italian Institute of Technology, Via Arnesano 16, 73100 Lecce, Italy.
Abstract:
The recombinant production of a novel chimeric polyprotein is described. The new protein contains either wild-type beta(2)-microglobulin (beta(2)m) or its truncated variant (DeltaN6 beta(2)m) (see picture). Structural characterization is achieved by means of single-molecule force spectroscopy studies of specific beta(2)m regions which could be involved in amyloidogenesis.
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