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Published on: February 10, 2014
Surface analysis of commercially pure titanium implant retrieved from rat bone. Part 1: initial biological response
Kouichi Watanabe1, Seigo Okawa, Mitugu Kanatani
1Division of Biomaterial Science, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, 5274 Gakkoucho-dori 2, Chuo-ku, Niigata 951-8514, Japan. watakou@dent.niigata-u.ac.jp
Abstract:
To gain insight on the early biological response to commercial pure titanium (cpTi), the surface properties of cpTi implants retrieved from rat bone were examined by X-ray photoelectron spectroscopy (XPS). To this end, semi-cylindrical bullets, 1.1 mm in diameter and 3.5 mm in length, were implanted into the femurs of Wistar rats and then retrieved after either 3 hours or 7 days. Regardless of implantation interval, elements of Ti, O, C, and N were observed on the retrieved implants and that the thickness of the adsorbed film (mainly protein) was estimated to be about 2.5 nm. Small amounts of both Ca and P were also detected, whereby the Ca/P atomic ratios after 3 hours and 7 days were very small compared to that of hydroxyapatite. Furthermore, no correlation was found between the Ca and P distributions in the element maps. In conclusion, no calcium phosphate compounds were formed on the implant in vivo after 7 days.
