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Related Concept Videos

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells01:18

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
Glial Cells01:04

Glial Cells

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Related Experiment Video

Updated: Jun 22, 2026

A 3D Spheroid Model for Glioblastoma
07:40

A 3D Spheroid Model for Glioblastoma

Published on: April 9, 2020

Glioma stem cells: better flat than round.

Kevin Woolard1, Howard A Fine

  • 1Neuro-Oncology Branch, National Cancer Institute, National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.

Cell Stem Cell
|June 6, 2009
PubMed
Summary

Researchers developed an efficient new method for deriving glioma-initiating cell lines. This protocol may improve the isolation and clinical use of these crucial cancer stem cells.

Area of Science:

  • Neuro-oncology
  • Cancer Stem Cell Biology
  • Cell Culture Techniques

Background:

  • Glioma-initiating cells (GIC) or glioma stem cells (GSC) are critical for brain tumor growth and recurrence.
  • Current in vitro methods, such as spheroid assays, for GIC/GSC isolation are inefficient and cumbersome.
  • Improved methods are needed for reliable GIC/GSC expansion for research and therapeutic development.

Discussion:

  • The study introduces a novel protocol for efficient GIC/GSC line derivation.
  • This new method addresses the limitations of traditional spheroid assays.
  • The protocol facilitates the generation of more robust GIC/GSC cultures.

Key Insights:

  • A streamlined protocol significantly enhances the efficiency of GIC/GSC line derivation.

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Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
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Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

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Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Published on: February 24, 2021

  • The improved method simplifies the isolation and expansion of these critical cell populations.
  • This advancement holds promise for overcoming previous bottlenecks in GIC/GSC research.

    • Outlook:

    • The efficient derivation protocol could accelerate the study of GIC/GSC biology.
    • This advancement may lead to improved preclinical models for brain tumor research.
    • Enhanced GIC/GSC isolation could facilitate their clinical application in targeted therapies.