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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

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Related Experiment Video

Updated: Jun 22, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Mdm2-mediated ubiquitylation: p53 and beyond.

J-C Marine1, G Lozano

  • 1Laboratory For Molecular Cancer Biology, VIB-UGent, Ghent B-9052, Belgium. chris.marine@dmbr.ugent.be

Cell Death and Differentiation
|June 6, 2009
PubMed
Summary

The Mdm2 oncoprotein targets the p53 tumor suppressor for degradation. This review covers new insights into Mdm2-p53 interactions and Mdm2

Area of Science:

  • Oncology
  • Molecular Biology
  • Drug Discovery

Background:

  • The Really Interesting Genes (RING)-finger-containing protein Mdm2 is a key regulator of the tumor suppressor p53.
  • Mdm2 promotes p53 ubiquitylation and proteasomal degradation, impacting cell cycle control and cancer development.
  • Understanding Mdm2-p53 interactions is crucial for developing targeted cancer therapies.

Purpose of the Study:

  • To review recent advances in understanding Mdm2-mediated regulation of p53.
  • To explore the regulation of physical and functional interactions between Mdm2 and p53.
  • To discuss the p53-independent functions of Mdm2 and their therapeutic implications.

Main Methods:

  • Literature review of recent scientific reports and genetic data.

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Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model
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Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model

Published on: April 17, 2026

Detection of Protein Ubiquitination
09:00

Detection of Protein Ubiquitination

Published on: August 19, 2009

Related Experiment Videos

Last Updated: Jun 22, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model
09:00

Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model

Published on: April 17, 2026

Detection of Protein Ubiquitination
09:00

Detection of Protein Ubiquitination

Published on: August 19, 2009

  • Analysis of molecular mechanisms underlying Mdm2-p53 interactions.
  • Evaluation of genetic evidence for p53-independent Mdm2 roles.

    • Main Results:

    • Novel insights into Mdm2-mediated regulation of p53 have been reported.
    • The regulation of Mdm2-p53 physical and functional interactions is increasingly understood.
    • Genetic data confirm a p53-independent role for Mdm2.

    Conclusions:

    • Advances in Mdm2 and p53 research offer new perspectives on cancer biology.
    • The identified Mdm2 functions, including p53-dependent and -independent roles, present promising avenues for novel cancer therapeutics.
    • Targeting Mdm2 interactions could lead to effective cancer treatment strategies.