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Updated: Jun 22, 2026

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
Published on: April 25, 2025
Time resolved fluorescence polarization anisotropy of carbonaceous particles produced in combustion systems
Abstract:
The size of nanometric carbonaceous particles produced in various combustion systems is determined by means of time resolved fluorescence polarization anisotropy (TRFPA). We also compare the performances of two different experimental implementations of thetechnique, which are complementary in terms of cost, simplicity and resolution. Both methods are first employed on standard molecules to demonstrate the reliability of the results. A study of the sizes of nanometric particles collected at the exhaust of diesel and gasoline vehicle engine, as well as from controlled laminar flames is presented. The high sensitivity (0.04 nm) achieved with the use of a streak camera as detector makes the TRFPA technique particularly suitable for characterizing nanometric particles.
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