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Updated: Jun 22, 2026

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
Published on: July 31, 2010
A fundamental introduction to ion mobility mass spectrometry applied to the analysis of biomolecules
Guido F Verbeck1, Brandon T Ruotolo, Holly A Sawyer
1The Laboratory for Biological Mass Spectrometry, Texas A&M University, College Station, Texas, USA.
Abstract:
Chemists are constantly striving for techniques that add dimensions of orthogonality with increased throughput and sample complexity. Ion mobility spectrometry (IM) is a gas-phase separation method that adds new dimensions to mass spectrometry (MS). IM separates gas-phase ions based on their collision cross-section and can be coupled with time-of-flight (TOF) mass spectrometry to yield a powerful tool used in the identification and characterization of proteins and peptides. A fundamental introduction to IM is presented with a focus on resolution, sensitivity, and orthogonality coupled with TOF-MS.
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