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Related Experiment Video

Updated: Jun 22, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

High-throughput sample preparation for protein or peptide structural characterization.

D G Sheer1, A M Pitt

  • 1Millipore Corporation, Danvers, MA, USA.

Journal of Biomolecular Techniques : JBT
|June 6, 2009
PubMed
Summary
This summary is machine-generated.

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Efficient sample preparation for high-performance liquid chromatography (HPLC) and mass spectrometry is crucial. MultiScreen 96-well filter plates offer a high-throughput solution for biomolecule analysis, improving speed and reproducibility.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Proteomics

Background:

  • High-performance liquid chromatography (HPLC) coupled with mass spectrometry is essential for biomolecular identification.
  • Rapid technological advancements necessitate efficient, high-throughput sample preparation methods.
  • Current sample preparation techniques can be a bottleneck in biomolecular analysis workflows.

Purpose of the Study:

  • To evaluate the efficiency of MultiScreen 96-well filter plates for high-throughput sample preparation.
  • To assess the performance of various chromatographic materials (C18, silica, divinylbenzene) in these plates.
  • To demonstrate the utility of these mini-columns for sample clean-up in HPLC and mass spectrometry.

Main Methods:

  • Testing of C18 resin, silica, and sulfonated divinylbenzene particles in MultiScreen 96-well filter plates.

Related Experiment Videos

Last Updated: Jun 22, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

  • Utilizing the Multiscreen Column Loader for simultaneous, uniform loading of samples.
  • Analysis of sample recovery and well-to-well reproducibility using HPLC.

    • Main Results:

    • MultiScreen filter plates efficiently removed salts and detergents, ensuring excellent sample recovery.
    • High well-to-well reproducibility was observed during analyte adsorption, washing, and elution.
    • Effective sample clean-up was demonstrated for proteins, peptides, and proteolytic digests.

    Conclusions:

    • MultiScreen 96-well filter plates provide a convenient and efficient solution for high-throughput sample preparation in HPLC and mass spectrometry.
    • The system demonstrates excellent reproducibility and sample recovery, addressing a critical bottleneck in biomolecular analysis.
    • These mini-columns are valuable tools for sample clean-up, enhancing the overall efficiency of proteomic workflows.