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Updated: Jun 22, 2026
Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection
Published on: February 28, 2021
Methods used to study respiratory virus infection
Emilio Flaño1, Nancy A Jewell, Russell K Durbin
1Center for Vaccines and Immunity, The Research Institute at Nationwide Children's Hospital, Ohio State University, Columbus, Ohio, USA.
Abstract:
This unit describes protocols for infecting the mouse respiratory tract, and assaying virus replication and host response in the lung. Respiratory infections are the leading cause of acute illness worldwide, affecting mostly infants and children in developing countries. The purpose of this unit is to provide a basic strategy and protocols to study the pathogenesis and immunology of respiratory virus infection using the mouse as an animal model. The procedures include: (1) basic techniques for mouse infection, tissue sampling, and preservation, (2) determination of viral titers, isolation and analysis of lymphocytes and dendritic cells using flow-cytometry, and (3) lung histology, immunohistochemistry, and in situ hybridization.
Insights
This study outlines mouse models for respiratory virus infections, detailing methods to analyze viral replication and the host immune response in the lungs. These protocols aid in understanding respiratory disease pathogenesis and immunology.
Area of Science:
- Immunology
- Virology
- Pathogenesis
Background:
- Respiratory infections represent a significant global health burden, particularly in developing nations, impacting infants and children.
- Understanding the pathogenesis and immunology of respiratory viruses is crucial for developing effective interventions.
Purpose of the Study:
- To establish a standardized mouse model strategy for studying respiratory virus infections.
- To provide detailed protocols for assessing viral replication and host immune responses in the lung.
Main Methods:
- Mouse infection models and tissue sampling techniques.
- Viral titer determination, lymphocyte and dendritic cell isolation via flow cytometry.
- Lung histology, immunohistochemistry, and in situ hybridization for detailed analysis.
Main Results:
- Established protocols for reproducible mouse infection and sample collection.
- Enabled quantitative assessment of viral load and immune cell populations.
- Facilitated detailed spatial and cellular analysis of lung tissue.
Conclusions:
- The described protocols provide a robust framework for investigating respiratory virus pathogenesis and immunology in a mouse model.
- This approach is essential for advancing research into respiratory diseases and informing public health strategies.
