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Related Concept Videos

Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...

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Related Experiment Video

Updated: Jun 22, 2026

Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection
07:36

Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection

Published on: February 28, 2021

Methods used to study respiratory virus infection.

Emilio Flaño1, Nancy A Jewell, Russell K Durbin

  • 1Center for Vaccines and Immunity, The Research Institute at Nationwide Children's Hospital, Ohio State University, Columbus, Ohio, USA.

Current Protocols in Cell Biology
|June 6, 2009
PubMed
Summary

This study outlines mouse models for respiratory virus infections, detailing methods to analyze viral replication and the host immune response in the lungs. These protocols aid in understanding respiratory disease pathogenesis and immunology.

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An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
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Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
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Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

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Last Updated: Jun 22, 2026

Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection
07:36

Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection

Published on: February 28, 2021

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
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An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

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Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

Area of Science:

  • Immunology
  • Virology
  • Pathogenesis

Background:

  • Respiratory infections represent a significant global health burden, particularly in developing nations, impacting infants and children.
  • Understanding the pathogenesis and immunology of respiratory viruses is crucial for developing effective interventions.

Purpose of the Study:

  • To establish a standardized mouse model strategy for studying respiratory virus infections.
  • To provide detailed protocols for assessing viral replication and host immune responses in the lung.

Main Methods:

  • Mouse infection models and tissue sampling techniques.
  • Viral titer determination, lymphocyte and dendritic cell isolation via flow cytometry.
  • Lung histology, immunohistochemistry, and in situ hybridization for detailed analysis.

Main Results:

  • Established protocols for reproducible mouse infection and sample collection.
  • Enabled quantitative assessment of viral load and immune cell populations.
  • Facilitated detailed spatial and cellular analysis of lung tissue.

Conclusions:

  • The described protocols provide a robust framework for investigating respiratory virus pathogenesis and immunology in a mouse model.
  • This approach is essential for advancing research into respiratory diseases and informing public health strategies.