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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jun 22, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

[Fluorescent linear labelling method for biochip applications].

Dapeng Ding1, Wenli Ma, Ying Zhang

  • 1Institute of Genetic Engineering, Nanfang Medical University, Guangzhou 510515, China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi
|June 9, 2009
PubMed
Summary
This summary is machine-generated.

The Fluorescent Linear Labelling technique offers a robust, single-tube method for amplifying RNA. This efficient process enhances transcript abundance for biochip hybridization, providing reliable data.

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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Context:

  • Oligonucleotide biochips require precise sample preparation for accurate gene expression analysis.
  • Traditional labelling methods can suffer from low amplification yields and loss of transcriptomic information.

Purpose:

  • To introduce and evaluate the Fluorescent Linear Labelling technique for enhanced biochip hybridization.
  • To provide a robust and efficient method for amplifying and labelling RNA samples.

Summary:

  • The Fluorescent Linear Labelling technique synthesizes antisense cRNA from cDNA derived from total RNA.
  • Utilizes T7 RNA polymerase for linear amplification, achieving 50- to 100-fold increases in labelled material.
  • The method preserves transcript abundance information, crucial for accurate biochip hybridization.

Impact:

  • Enables more sensitive and reliable gene expression profiling using oligonucleotide biochips.
  • Offers a significant improvement over existing methods in terms of amplification efficiency and data integrity.
  • Facilitates high-throughput analysis and discovery in various biological research fields.