Related Experiment Video
Updated: Jun 22, 2026

Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals
Published on: February 20, 2020
Total body water changes after an exercise intervention tracked using bioimpedance spectroscopy: a deuterium oxide
Jordan R Moon1, Abbie E Smith, Sarah E Tobkin
1Metabolic and Body Composition Laboratories, University of Oklahoma, Norman, OK 73019, USA.
Background & Aims:
Bioimpedance spectroscopy (BIS) for the estimation of total body water (TBW) has advantages over isotope dilution techniques, including cost, portability, and ease of use. The aim of the current study was to determine the validity of a BIS device (Imp SFB7) for tracking changes in overfat and obese individuals.
Methods:
Sixty overfat and obese men and women (27+/-8 yr, 33.41+/-3.81%fat) participated in the study. TBW was estimated using BIS and deuterium oxide (D(2)O) before and after the ten-week intervention.
Results:
Pre-and post-intervention BIS TBW estimations errors increased as body mass index (BMI), fat mass (FM), and fat-free mass (FFM) increased (p<0.05). Delta values were more accurate than pre- and post-TBW estimations (total error=1.45 L). Age significantly influenced pre- and post-TBW errors (p<0.05). Therefore, a regression equation was developed to correct for the pre- and post-BIS-estimated TBW errors: D(2)O TBW=11.478+0.743(BIS TBW)-2.429(Gender), (Men=1, Women=2).
Conclusions:
BIS can be considered an accurate tool for tracking changes in TBW regardless of variations in BMI, FM, FFM, or age in both overfat and obese men and women (BMI>24). Employing a BIS TBW equation is suggested over the development of resistivity coefficients based on BMI, FM, FFM or age.
More Related Videos
10:21Evaluation of Hydration Status by Bioelectrical Impedance Vector Analysis in Patients with Ischemic Heart Disease Undergoing Exercise Stress Test
Published on: September 22, 2023
07:44Evaluation of Changes in Hydration and Body Cell Mass with Bioelectrical Impedance Analysis after Exercise Program for Rheumatoid Arthritis Patients
Published on: July 14, 2023
Related Concept Videos
Body Water Content and Fluid Compartments
Strength and Heat of Hydration
The heat of hydration for each cement compound is significant; for instance, tricalcium aluminate (C3A) and...
Disorder of Water Balance
Dehydration
Dehydration occurs when the body loses fluids (particularly water).
Causes:
The major causes of dehydration include excessive sweating, fever, vomiting, diarrhea, and diuresis.
Signs and Symptoms:
Symptoms primarily include intense...