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Updated: Jun 22, 2026
Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles
Published on: May 6, 2012
Headspace solid-phase microextraction gas chromatography-mass spectrometry analysis of Eupatorium odoratum extract as
Shufen Cui1, Shuo Tan, Gangfeng Ouyang
1Department of Biological Applied Engineering, Shenzhen Polytechnic, Shenzhen 518055, China. shufencui@163.com
Abstract:
Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) analysis was used to study volatile and semi-volatile compounds emitted by the Eupatorium odoratum (E. odoratum) extract. Variables of HS-SPME such as the type of SPME fiber, extraction time and temperature, incubation time, desorption time and temperature have been optimized. Optimized conditions were obtained by the use of divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber, 5 min/20 min incubation/extraction time at 65 degrees C, 5 min desorption time at 260 degrees C. Using three different polar chromatographic columns to get retention index and mass spectrometry data, 99 volatile and semi-volatile compounds were tentatively identified in the E. odoratum extract. This study has identified the promising source of E. odoratum oviposition repellent.
