Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fungal Phylum Microsporidia

Fungal Phylum Microsporidia

Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prevalence and molecular identification of protozoan parasites in cancer patients in Jordan.

Journal of infection in developing countries·2026

Same author

Low Vitamin D and C Levels are Associated with Increased Disease Activity in Jordanian RA and SLE Patients: A Case-Control Study

Endocrine, metabolic & immune disorders drug targets·2026

Same author

The status of strongyloidiasis in the Mediterranean countries.

Journal of infection in developing countries·2025

Same author

Microphysiological gut-on-chip enables extended <i>in vitro</i> development of <i>Cryptosporidium hominis</i>.

Frontiers in cellular and infection microbiology·2025

Same author

The role of vitamins D, B12, C, and K in modulating inflammation and disease management in rheumatoid arthritis: a comprehensive review.

Clinical rheumatology·2024

Same author

Comparison of in vitro growth characteristics of Cryptosporidium hominis (IdA15G1) and Cryptosporidium parvum (Iowa-IIaA17G2R1 and IIaA18G3R1).

Parasitology research·2023

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection

Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection

Published on: September 14, 2019

Cryptosporidium: new developments in cell culture.

Nawal Hijjawi¹

¹Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, The Hashemite University, P.O. Box 150459, Zarqa 13115, Jordan. nhijjawi@hu.edu.jo

Experimental Parasitology

|June 9, 2009

Summary

Developing reproducible Cryptosporidium culture models is crucial for research. Recent advances enable long-term, cell-free cultivation, allowing the parasite

Area of Science:

Parasitology
Infectious Diseases
Cell Biology

Background:

Limited parasitic stages hinder Cryptosporidium research.
Developing a reproducible culture model is a key objective.
Previous methods faced challenges with host cell aging and overgrowth.

Purpose of the Study:

To review factors influencing in vitro Cryptosporidium culture success.
To discuss advances in cell-free culture systems.
To propose future optimization strategies for Cryptosporidium cultivation.

Main Methods:

Long-term culturing using pH modification, sub-culturing, and gamma irradiation.
Cell-free culture systems enabling parasite life cycle completion.
Improved visualization techniques for life cycle stages in vitro.

More Related Videos

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Published on: June 22, 2019

Related Experiment Videos

Last Updated: Jun 22, 2026

Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection

Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection

Published on: September 14, 2019

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Published on: June 22, 2019

Main Results:

Successful long-term cultivation of Cryptosporidium achieved.
Parasite life cycle completion demonstrated in cell-free medium.
Overcoming limitations of host cell-dependent cultures.

Conclusions:

Cell-free culture systems offer a viable alternative for Cryptosporidium research.
Optimized culture conditions are essential for reproducible results.
Further research can enhance cell-free culture efficiency and application.