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Updated: Jun 22, 2026

Site-Specific Lysine Lactylation via Genetic Code Expansion in E. coli and Mammalian Cells
Published on: February 24, 2026
Isolation and characterization of a novel lysine racemase from a soil metagenomic library
I-Chien Chen1, Wei-De Lin, Shin-Kuang Hsu
1Institute of Molecular Biology, College of Life Sciences, [corrected] National Chung Hsing University, Taichung, Taiwan.
Abstract:
A lysine racemase (lyr) gene was isolated from a soil metagenome by functional complementation for the first time by using Escherichia coli BCRC 51734 cells as the host and d-lysine as the selection agent. The lyr gene consisted of a 1,182-bp nucleotide sequence encoding a protein of 393 amino acids with a molecular mass of about 42.7 kDa. The enzyme exhibited higher specific activity toward lysine in the l-lysine-to-d-lysine direction than in the reverse reaction.

