[The modelling of the translocation of the intestinal microflora in conventional animals]

To study the mechanisms of the translocation of enteric microflora and to develop the corresponding prophylactic measures, experimental models are necessary. In this connection two methods of simulating translocation are proposed. One of these methods is reproduced in animals resuscitated from the state of apparent death caused by acute venous blood loss and the other method, in animals with acute renal insufficiency induced by the ligation of the middle part of both ureters. During the period from the first hours to two weeks after the state of apparent death enterobacteria penetrate into mesenteric lymph nodes and occasionally into the liver, spleen and blood. On day 3 after the ligation of the ureters enterobacteria can be isolated from the above-mentioned organs and the blood, while the kidneys remain sterile. The models are reproducible, exhibit adequate clinical manifestations of these terminal states, and permit the detailed investigation of the translocation mechanisms.