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Related Experiment Video

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Modeling Mucosal Candidiasis in Larval Zebrafish by Swimbladder Injection

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Published on: November 27, 2014

'Injecting' yeast.

Daniel Riveline¹, Paul Nurse

¹Laboratory of Yeast Genetics and Cell Biology, The Rockefeller University, New York, New York, USA. daniel.riveline@ujf-grenoble.fr

|June 9, 2009

Summary

This summary is machine-generated.

Researchers developed a new method to inject substances into yeast cells, overcoming the challenge of their rigid cell walls. This technique allows for genetic manipulation and study of cellular processes in fission yeast (Schizosaccharomyces pombe).

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Area of Science:

Cell Biology
Biophysics
Microfluidics

Background:

Yeast (Schizosaccharomyces pombe) is a vital genetic model organism.
The rigid yeast cell wall impedes direct intracellular manipulation, limiting experimental approaches.
Existing methods are insufficient for precise molecular delivery into yeast.

Purpose of the Study:

To establish a novel method for intracellular delivery into yeast cells.
To overcome the physical barrier posed by the yeast cell wall.
To enable functional studies of intracellular components in Schizosaccharomyces pombe.

Main Methods:

Utilized microfabricated channels to physically constrain individual yeast cells.
Employed a piezoelectric-driven unit to shear localized regions of the cell wall.
Introduced fluorescent probes and inhibitors into the cell interior.

Main Results:

The developed method successfully created transient pores in yeast cell walls, maintaining cell viability.
Intracellular delivery of fluorescent phalloidin confirmed the presence of actin patches post-procedure.
Disruption of the cytokinetic ring was observed upon blebbistatin injection, indicating successful delivery of the myosin II inhibitor.

Conclusions:

This technique provides a viable method for microinjection into yeast, previously a significant challenge.
The approach allows for the study of cellular dynamics and the effects of specific molecular interventions in Schizosaccharomyces pombe.
This breakthrough enhances the utility of yeast as a model system for cell biology research.