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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 22, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing.

A Esposito, T Oggier, H Gerritsen

    Optics Express
    |June 9, 2009
    PubMed
    Summary

    We present a cost-effective Fluorescence Lifetime Imaging Microscopy (FLIM) solution using a simple CCD/CMOS lock-in imager. This compact system, based on a time-of-flight detector, makes advanced FLIM more accessible for life science applications.

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    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

    Published on: February 9, 2012

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    Last Updated: Jun 22, 2026

    Fluorescence Lifetime Macro Imager for Biomedical Applications
    06:01

    Fluorescence Lifetime Macro Imager for Biomedical Applications

    Published on: April 7, 2023

    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
    09:45

    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

    Published on: February 9, 2012

    Area of Science:

    • Biophotonics and Life Sciences
    • Microscopy Techniques

    Background:

    • Fluorescence Lifetime Imaging Microscopy (FLIM) is a valuable tool in life sciences.
    • Wider adoption of FLIM is hindered by cost and complexity.
    • Existing FLIM systems often require specialized setups.

    Purpose of the Study:

    • To demonstrate a user-friendly and cost-effective FLIM system.
    • To adapt a time-of-flight detector for fluorescence lifetime measurements.
    • To explore potential for high-throughput FLIM applications.

    Main Methods:

    • Utilized a SwissRanger SR-2 time-of-flight detector, a CCD/CMOS lock-in imager.
    • Integrated the detector into a compact system for FLIM.
    • Explored potential combinations with light-emitting and laser diodes.

    Main Results:

    • Successfully implemented FLIM detection using a simple lock-in imager.
    • The SwissRanger SR-2 detector provides essential FLIM functionalities in a compact form.
    • The developed system shows promise for broader FLIM accessibility.

    Conclusions:

    • A compact and cost-effective FLIM system is achievable using readily available technology.
    • This approach can significantly lower the barrier to entry for FLIM adoption.
    • Future development could enable widespread FLIM use, including high-throughput screening.