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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 22, 2026

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
10:04

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

Stimulated parametric emission microscopy.

Keisuke Isobe, Shogo Kataoka, Rena Murase

    Optics Express
    |June 9, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new microscopy method using enhanced four-wave mixing (FWM) for detailed imaging. This technique successfully visualized both a liquid solution and a plant leaf with high resolution.

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    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

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    Published on: October 28, 2018

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    Published on: October 20, 2017

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    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    Area of Science:

    • Optics and Photonics
    • Biophotonics
    • Microscopy

    Background:

    • Four-wave mixing (FWM) is a nonlinear optical process.
    • Existing microscopy techniques have limitations in resolution and sensitivity.
    • Nonlinear optical microscopy offers advanced imaging capabilities.

    Purpose of the Study:

    • To introduce a novel microscopy technique utilizing enhanced four-wave mixing (FWM).
    • To leverage two-photon electronic resonance and stimulated emission for improved imaging.
    • To demonstrate the applicability of the technique for biological and chemical samples.

    Main Methods:

    • Implementation of a microscopy technique based on four-wave mixing (FWM).
    • Enhancement of FWM using two-photon electronic resonance (pump pulse) and stimulated emission (dump pulse).
    • Utilization of a Ti:sapphire laser and an optical parametric oscillator as light sources.

    Main Results:

    • Successful acquisition of a one-dimensional image of an ethanol-thinned Coumarin 120 solution.
    • Generation of a two-dimensional image of a *Camellia sinensis* (tea plant) leaf.
    • Demonstration of the technique's capability for imaging diverse samples.

    Conclusions:

    • The proposed FWM-based microscopy technique is effective for high-resolution imaging.
    • The method shows potential for various applications in chemical analysis and biological imaging.
    • This novel approach advances nonlinear optical microscopy capabilities.