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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...

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Related Experiment Video

Updated: Jun 22, 2026

Gene Trapping Using Gal4 in Zebrafish
13:34

Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

Gene trap: knockout on the fast lane.

Melanie Ullrich1, Kai Schuh

  • 1Institute of Physiology I, University of Wuerzburg, Wuerzburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|June 9, 2009
PubMed
Summary

Gene trapping offers a powerful alternative to gene targeting for studying gene function and promoter activity. This method utilizes available embryonic stem cell clones for efficient gene function ablation and analysis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Gene trapping is an effective technique for gene function ablation and in vivo promoter analysis.
  • It is less common than traditional gene targeting but provides unique advantages.
  • The International Gene Trap Consortium (IGTC) offers a wide array of embryonic stem cell clones with diverse trapped genes.

Purpose of the Study:

  • To provide a comprehensive guide to utilizing gene trapping strategies.
  • To detail methods for identifying and confirming suitable gene trap stem cell clones.
  • To outline protocols for characterizing insertion sites and reporter gene activity.

Main Methods:

  • Utilizing BLAST searches to identify appropriate embryonic stem cell clones.

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Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
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Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

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Last Updated: Jun 22, 2026

Gene Trapping Using Gal4 in Zebrafish
13:34

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Published on: September 29, 2013

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

  • Confirming vector insertion via RT-PCR and X-Gal staining.
  • Characterizing insertion sites for PCR-based genotyping.
  • Following the activity of beta-galactosidase reporters.

    • Main Results:

    • Established protocols for gene trap stem cell clone selection and validation.
    • Detailed methods for precise insertion site characterization.
    • Provided guidance on analyzing gene promoter activity using reporter genes.

    Conclusions:

    • Gene trapping is a valuable, albeit underutilized, tool in genetic research.
    • The IGTC resource facilitates efficient gene function studies.
    • This chapter provides practical protocols for implementing gene trapping strategies.