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Updated: Jun 22, 2026
Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
Published on: October 8, 2015
[An investigation of BRAF mutation in papillary thyroid carcinoma and its clinical value]
Ri-xiang Gong1, Yang Zhou, Shu-hua Luo
1Department of Thyroid and Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041 PR China.
Objective:
To study the relationship between BRAF mutation and clinicopathological features in papillary thyroid carcinoma (PTC).
Methods:
Fresh samples were examined for the presence of BRAF mutations in 43 patients with PTC and 20 patients with non-PTC thyroid disease and 40 normal thyroid tissues by polymerase chain reaction (PCR) and direct sequencing. The relationship between BRAF mutation and clinicopathological features was studied.
Results:
BRAF mutation was detected in 39.5% (17/43) of PTC samples, in 0 of non-PTC thyroid disease samples and normal thyroid tissues. Significant association was seen between BRAF mutation and both extrathyroidal invasion and cervical lymph node metastasis (P<0.05, P<0.05). There was no significant relationship between BRAF mutation and gender, age at the time of diagnosis, tumor size and distant metastasis.
Conclusion:
BRAF mutation is associated with extrathyroidal invasion and lymph node metastasis. It may increase the ability of invasion and metastasis of PTC and have influence on prognosis.
