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Updated: Jun 22, 2026

Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates
Published on: January 5, 2024
The dynamic side of the Hofmeister effect: a single-molecule nanopore study of specific complex formation
Philip A Gurnev1, Daniel Harries, V Adrian Parsegian
1Laboratory of Physical and Structural Biology, Program in Physical Biology, NICHD, National Institutes of Health, 9000 Rockville Pike, Bldg. 9, Bethesda, MD 20892, USA.
Abstract:
Beyond measurements of equilibria: An alpha-hemolysin nanopore is used as a single-molecule sensor to follow the effects of different salts on the complexation reaction of gamma-cyclodextrin and adamantane carboxylate (see picture). The kinetics underlying the dynamic equilibrium are studied to reveal qualitatively different dynamic actions of various cosolute salts.
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