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Updated: Jun 22, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Preparation and identification of monoclonal antibodies to staphylococcal enterotoxin I]
Jun-Ying Xu1, Ding Ding, Hong-Ying Sun
1Institute of Pharmacology, Toxicology and Biological Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Objective:
To prepare and identify monoclonal antibodies against staphylococcal enterotoxin I (SEI).
Methods:
Spleen cells obtained from mice immunized with the SEI protein were fused with the myeloma cells (SP2/0). Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) and the stable monoclonal hybridomas were isolated by limiting dilution at least three times. The characters of purified monoclonal antibodies were identified by indirect ELISA and Western blotting.
Result:
The monoclonal antibodies secreted by two hybridomas 8F7 and D8 belonged to IgG(2b) and IgG(1) subtypes. Both had high titer and specificity with no cross reaction to SEG, SEE and SEC.
Conclusion:
The monoclonal antibodies against SEI has been successfully prepared and identified in this study.
