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Updated: Jun 22, 2026
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
[Study on enzyme-solvent extraction process of flavonoids from leaves of Ginkgo biloba]
Na-na Ouyang1, Xiang-zhou Li, Zheng Luo
1Materials Science and Engineering Institute, Central South University of Forestry and Technology, Changsha 410004, China. oynn@netease.com
Objective:
To study the optimum enzyme-solvent extraction conditions of flavonoids from leaves of Ginkgo biloba.
Methods:
To optimize the process by single factor experiments and orthogonal test according to the yield of flavonoids.
Results:
The optimum extraction conditions were established as follows: pH 5.7, the concentration of enzyme 0.4 mg/mL, enzymatic hydrolysis temperature 45 degrees C, enzymatic hydrolysis time 2 h, the rate of liquid to material 20:1, extraction temperature 70 degrees C, extraction time 2 h, the concentration of ethanol 70%, the average yield of flavonoids from leaves of Ginkgo biloba was 3.62%.
Conclusion:
The method is simple and can increase material utility effectively.
