Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel CDK-independent function of CDC25 phosphatases in mRNA translation.

EMBO reports·2026
Same author

Proteomic analysis of malignant ascites and its impact on ovarian cancer spheroids.

Clinical proteomics·2026
Same author

The genetic driver of Acute Necrotizing Encephalopathy, RANBP2, regulates the inflammatory response to Influenza A virus infection.

Nature communications·2026
Same author

Genotype-Phenotype Correlations in Recessive Dystrophic Epidermolysis Bullosa: A Systematic Review.

JAMA dermatology·2026
Same author

Scientists' warning: we must change paradigm for a revolution in toxicology and world food supply.

Environmental sciences Europe·2026
Same author

[ANEmone : la 1<sup>re</sup> conférence Science & Familles sur l'encéphalopathie nécrosante aiguë suscite l'espoir pour une maladie génétique ultra-rare].

Virologie (Montrouge, France)·2025

Related Experiment Video

Updated: Jun 22, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.

Stefania Millevoi1, Adrien Decorsière, Clarisse Loulergue

  • 1INSERM, U563, Toulouse, Université de Toulouse, UPS, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France. stefania.millevoi@inserm.fr

Nucleic Acids Research
|June 10, 2009
PubMed
Summary

Polypyrimidine tract-binding protein (PTB) enhances mRNA 3' end processing by recruiting hnRNP H. This interaction stimulates polyadenylation, revealing a coordinated regulation mechanism involving splicing factors and polyadenylation signals.

More Related Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

Related Experiment Videos

Last Updated: Jun 22, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

Area of Science:

  • Molecular Biology
  • RNA Processing
  • Gene Regulation

Background:

  • Polypyrimidine tract-binding protein (PTB) is known as a splicing regulator.
  • PTB also positively influences pre-mRNA 3' end processing at the polyadenylation (pA) signal.
  • The precise mechanism of PTB's stimulatory role in mRNA 3' end formation requires further elucidation.

Purpose of the Study:

  • To investigate the mechanism by which PTB stimulates mRNA 3' end processing.
  • To identify the protein interactions and regulatory pathways involved in PTB's function.
  • To understand how PTB influences polyadenylation signal recognition.

Main Methods:

  • Investigated PTB binding to the human beta-globin (HBB) 3' untranslated region (UTR).
  • Assessed the impact of a processing-inactivating mutation on PTB binding.
  • Performed in vitro cleavage and polyadenylation assays.
  • Examined the recruitment of hnRNP H by PTB to G-rich sequences.
  • Studied the interaction between hnRNP H and poly(A) polymerase.

Main Results:

  • PTB binding to the HBB 3' UTR was abrogated by a specific mutation.
  • PTB was shown to promote both in vitro 3' end cleavage and polyadenylation.
  • PTB directly recruits the splicing factor hnRNP H to G-rich sequences near pA signals.
  • hnRNP H binding stimulates polyadenylation via direct interaction with poly(A) polymerase.

Conclusions:

  • PTB plays a crucial role in promoting mRNA 3' end formation.
  • PTB facilitates polyadenylation by recruiting hnRNP H, which interacts with poly(A) polymerase.
  • These findings demonstrate a coordinated regulation of pA signal recognition by splicing factors bound to auxiliary elements.