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Related Experiment Video

Updated: Jun 22, 2026

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

A 0.4-mm-diameter probe for nonlinear optical imaging.

Hongchun Bao¹, Min Gu

¹Centre for Micro-Photonics, Faculty of Engineering & Industrial Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia.

|June 10, 2009

Summary

This summary is machine-generated.

A novel 0.4 mm miniaturized probe enables clear two-photon fluorescence imaging. This device focuses laser beams for high-resolution microscopy, advancing in-vivo imaging capabilities.

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Implementation of a Reference Interferometer for Nanodetection

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Published on: April 26, 2014

Area of Science:

Optics and Photonics
Biomedical Imaging
Materials Science

Background:

Two-photon-excited fluorescence imaging offers deep tissue penetration and high resolution.
Miniaturized probes are crucial for in-vivo imaging and minimally invasive procedures.
Conventional probes can be bulky, limiting their application in small biological structures.

Purpose of the Study:

To develop a miniaturized fiber-optic probe for two-photon-excited fluorescence imaging.
To achieve direct focusing of femtosecond pulsed laser beams for imaging applications.
To create a probe with a small diameter suitable for minimally invasive procedures.

Main Methods:

Manufacturing a miniaturized probe by collapsing air holes in a double-clad photonic crystal fiber (DCPCF).
Forming a lens at the DCPCF tip using electric arc discharging from a fusion splicer.
Utilizing the lensed DCPCF to deliver and focus a femtosecond pulsed laser beam.

Main Results:

A 0.4 mm diameter miniaturized probe was successfully fabricated.
The probe achieved a numerical aperture of 0.12.
A focal spot size of 6 micrometers, near the diffraction limit, was obtained.
Clear two-photon-excited fluorescence images of 10-micrometer fluorescent microspheres were acquired.

Conclusions:

The developed miniaturized probe is effective for two-photon-excited fluorescence imaging.
The probe's small size and focusing capability are suitable for advanced microscopy.
This technology holds potential for in-vivo biological imaging and diagnostics.