Related Experiment Video
Updated: Jun 22, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
In vitro cytotoxicity of orthodontic primers
Vincenzo D'Antò1, Gianrico Spagnuolo, Ilaria Polito
1Institue of Composite and Biomedical Materials - Naples, Italy.
Objectives:
The aim of this study was to evaluate the cytotoxicity of four orthodontic primers: Transbond XT and Transbond MIP (3M, USA), Eagle Fluorsure (American Orthodontics, USA) and Ortho Solo (Ormco, USA).
Materials And Methods:
Balb 3T3 cells were exposed to different concentrations of primers (0-0.25 mg/ml). Mitochondrial dehydrogenase activity was evaluated by MTT assay and cell necrosis was measured by flow cytometry (propidium iodide staining).
Results:
All the materials decreased cell viability in a dose related manner. Cytotoxicity of orthodontic primers based on concentrations which caused a 50% decrease of mitochondrial activity was ranked as follows: Transbond XT (45.57 mg/ml) > Eagle Fluorsure (49.27 mg/ml) > Transbond MIP (64.35 mg/ml) > Ortho solo (70.09 mg/ml).
Conclusions:
Our results suggest that the cytotoxic potencies demonstrated by orthodontic primers might be of clinical relevance since they disturbed cell metabolism and induced cell death in monolayer cultures.
