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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S rDNA sequences
1Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China.
Abstract:
In this study, the relationships of five varieties in Perilla frutescens (L.) Britton were analyzed. All the varieties showed the same chromosome number 2n = 40, and the banding patterns of PI-DAPI staining and the FISH results of 45S rDNA probe were on the same site of the chromosome pair. The 5S rRNA gene spacers were ranged from 413 bp in P. frutescens var. purpurascens and 408 bp in var. frutescens. The varieties analyzed here were clustered into two clades according to the phylogenetic analysis. The sequence identity of 96% between var. auriculato-dentata and var. arguta suggested they could be considered the same variety.
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