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Updated: Jun 22, 2026

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Published on: October 1, 2012

Genome-wide gene expression profiling of SCID mice with T-cell-mediated Colitis.

D Brudzewsky¹, A E Pedersen, M H Claesson

¹Wilhelm Johannsen Centre for Functional Genome Research, Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark.

Scandinavian Journal of Immunology

|June 11, 2009

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This study uses a mouse model to identify genes involved in inflammatory bowel disease (IBD) pathogenesis. Findings highlight inflammatory pathways and potential new molecular markers for human IBD.

Area of Science:

Immunology
Gastroenterology
Genomics

Background:

Inflammatory bowel disease (IBD) is a complex condition with unknown causes.
Identifying key genes and pathways is crucial for understanding IBD pathogenesis and finding new markers.

Purpose of the Study:

To utilize a murine model of IBD to discover critical pathways and genes involved in disease development.
To identify potential molecular markers for human IBD through genome-wide gene expression analysis.

Main Methods:

Adoptive transfer of activated CD4(+) T cells into severe combined immunodeficient (SCID) mice to induce colitis.
Genome-wide gene expression analysis of rectal tissue using Affymetrix MOE430A arrays.
Histological scoring to correlate gene expression changes with inflammation levels.

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Published on: April 21, 2015

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Last Updated: Jun 22, 2026

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Published on: October 1, 2012

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4⁺CD45RB^high T Cells into Immunodeficient Mice

Published on: April 21, 2015

Main Results:

Significant differential gene expression (P = 0.00001) observed in 152 genes between control and colitis mice.
Overrepresentation of genes involved in inflammatory processes, including S100A proteins and chemokines.
Gene expression patterns correlated with distinct levels of rectal inflammation.

Conclusions:

The SCID mouse model effectively replicates human IBD, serving as a valuable tool for research.
Candidate genes and molecular markers for chronic inflammation in human IBD were identified.