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Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
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Published on: April 23, 2017

Multivalent ligand-receptor binding on supported lipid bilayers.

Hyunsook Jung1, Aaron D Robison, Paul S Cremer

  • 1Department of Chemistry, Texas A&M University, College Station, 77843-3012, USA.

Journal of Structural Biology
|June 11, 2009
PubMed
Summary

Supported lipid bilayers enable study of multivalent protein-ligand interactions. Ligand presentation significantly impacts binding affinity, altering apparent dissociation constants (K(D)) by orders of magnitude.

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12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Area of Science:

  • Biochemistry
  • Biophysics
  • Materials Science

Background:

  • Fluid supported lipid bilayers facilitate study of multivalent protein-ligand interactions.
  • Membrane fluidity allows ligand rearrangement to optimize binding.
  • Supported lipid bilayers are excellent platforms for studying molecular interactions.

Purpose of the Study:

  • To review findings on multivalent protein-ligand interactions using supported lipid bilayers.
  • To investigate the impact of ligand density and presentation on binding affinity.
  • To determine equilibrium dissociation constants (K(D)) for these systems.

Main Methods:

  • Utilizing supported lipid bilayer-coated microfluidic platforms.
  • Employing total internal reflection fluorescence microscopy (TIRFM).
  • High-throughput, on-chip analysis for thermodynamic data acquisition.

Main Results:

  • Increasing ligand density yields modest changes (up to one order of magnitude) in apparent K(D).
  • Lipid bilayer-conjugated ligand presentation significantly impacts binding affinity.
  • Altering ligand conjugation can change apparent K(D) by at least three orders of magnitude.

Conclusions:

  • Ligand availability and presentation are critical factors in multivalent binding.
  • Supported lipid bilayers offer a sensitive platform for quantifying binding thermodynamics.
  • This approach requires minimal sample volumes for accurate K(D) determination.