Lumbar lateral motor columns and hindlimbs of two Xenopus laevis chromosome mosaics

Two chromosome mosaic Xenopus laevis, one tadpole and one metamorphic animal, both with different sizes of neurons on the left and right sides of their brains and spinal cords, have left and right lumbar lateral motor columns (L-LMCs) of equal lengths but composed of strikingly different numbers of motoneurons (40% fewer motoneurons on the side composed of larger cells). One portion of the lumbar cord in the metamorphic animal is bilaterally symmetrical; the cells on both sides are small and the numbers of motoneurons per section are the same. The mosaics demonstrate that column length and motoneuron density (number per section) are, or can be, regulated bilaterally and that changing cell size affects factors controlling cell density but not column length. Except for the peripheral nerves, there is no evidence of any side-to-side differences in the hindlimb tissues. Whether the side-to-side difference in L-LMC motoneuron number in the stage 66 mosaic corresponds to any feature of the hindlimbs is unknown, but similar side-to-side differences in an early and a late stage mosaic animal support the idea that whatever creates the initial number may also determine the final number of motoneurons.