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Updated: Jun 22, 2026

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
Published on: April 30, 2016
A mutation in the tomato DDB1 gene affects cell and chloroplast compartment size and CDT1 transcript
Nili Caspi1, Ilan Levin, Daniel A Chamovitz
1Institute of Plant Sciences; The Volcani Center; Bet Dagan, Israel.
Abstract:
Fruits harvested from tomato (Solanum lycopersicum) plants carrying mutations at the DAMAGED DNA BINDING PROTEIN 1 gene (SlDDB1; hp-1 and hp-1(w)) are characterized by significantly elevated levels of lycopene and several other phytonutrients. We hypothesize that the pleotropic effect on mutant Slddb1 are some general function of DDB1 protein on cell growth. The main objective of this research was to carry out functional analysis of the mutant SlDDB1 alleles both in-planta and ex-planta in comparison to their normal counterparts. Our major results indicate that: mutant Slddb1 seedlings exhibited delayed growth and smaller cell size, greater chloroplast density, smaller chloroplasts and higher concentration of chlorophyll.Cotyledons cells of Slddb1 mutant also displayed abnormal stomatal pattern, reduced content of CDT1 transcript and altered response to cytokinin. Some of these observations were previously described to be connected to defects in cell cycle control. Our results, coupled with former studies, also suggest that the CDD complex (composed of DDB1, DET1 and COP10) mediate the effect of light and cytokinin activity by possibly regulating the replication licensing factor CDT1 thus affecting both cell size and plastid multiplication.
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