Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probe and Assay Dependent Pharmacology of Dantrolene Analogues at the GPR35.

ACS medicinal chemistry letters·2026
Same author

Racial and Regional Differences of the Cheek for Facial Feminization: A Systematic Review.

The Journal of craniofacial surgery·2026
Same author

Multimodal opioid-free anesthesia containing esketamine versus opioid-based anesthesia: a systematic review and meta-analysis.

BMC anesthesiology·2026
Same author

Perceived stress and self-reported bruxism in medical and dental students: a cross-sectional study using the BruxScreen tool.

Oral surgery, oral medicine, oral pathology and oral radiology·2026
Same author

Efficacy, tolerability, and safety of 1.5L-lactulose as bowel preparation for patients with inflammatory bowel disease: a randomized, single-blinded trial.

Internal and emergency medicine·2026
Same author

Ellagic acid alleviates ulcerative colitis in a GPR35-dependent manner associated with SDH restoration.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026

Related Experiment Video

Updated: Jun 22, 2026

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

Resonant waveguide grating biosensor for whole-cell GPCR assays.

Ye Fang1, Ann M Ferrie, Elizabeth Tran

  • 1Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2009
PubMed
Summary

New label-free biosensor assays examine cellular systems and pharmacology of G protein-coupled receptors (GPCRs). These advanced assays enable high-throughput screening, potentially reducing drug discovery attrition rates.

More Related Videos

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
08:46

A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

Published on: December 2, 2022

Related Experiment Videos

Last Updated: Jun 22, 2026

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
08:46

A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

Published on: December 2, 2022

Area of Science:

  • Pharmacology
  • Cell biology
  • Biotechnology

Background:

  • Current G protein-coupled receptor (GPCR) drug discovery often uses biased artificial cell assays.
  • This limits the study of endogenous receptors in relevant physiological environments.

Purpose of the Study:

  • To develop novel label-free optical biosensor cellular assays.
  • To examine systems cell biology and pharmacology of endogenous GPCRs in native environments.

Main Methods:

  • Development of label-free optical biosensor cellular assays.
  • Application in high-throughput screening of GPCR ligands.
  • Utilizing physiologically and disease-relevant cellular environments.

Main Results:

  • Demonstrated capability to study endogenous receptors in native cellular environments.
  • Successfully performed high-throughput screening of pathway-biased ligands for the beta(2)-adrenergic receptor.
  • Showcased the utility of biosensor assays for systems pharmacology.

Conclusions:

  • Label-free biosensor assays offer a more comprehensive approach to GPCR drug discovery.
  • These assays can provide deeper insights into receptor behavior in relevant biological contexts.
  • Potential to significantly reduce attrition rates in drug development pipelines.