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Using reporter gene technologies to detect changes in cAMP as a result of GPCR activation

Daniel J Rodrigues1, David McLoughlin

  • 1Pfizer Global Research and Development, High Throughput Screening Centre of Emphasis, Sandwich, UK.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2009
PubMed

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...