[Structure and function of matrix metalloproteinases]
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Updated: Jun 22, 2026

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
Published on: March 30, 2022
Krzysztof Olszyński1, Małgorzata Zimowska
1Instytut Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego PAN, Pracownia Fizjologii Nerek i Płynów Ustrojowych, Warszawa.
Abstract:
The ECM remodeling is a finely orchestrated process that involves changes in expression of the ECM genes and the activity of proteinases to clear the previous ECM deposit. Among enzymes involved in ECM remodeling, zinc-dependent proteinases--matrix metalloproteinases (MMPs) are believed to be the ones of major importance. MMPs degrade the ECM components and regulate the bioavailability and/or activity of growth factors by cleavage of both matrix and non-matrix substrates or by mediating receptor turnover. This review introduces the members of the MMP family and discusses their domain structure and function, proenzyme activation and the mechanism of inhibition.
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