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The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
Published on: February 27, 2026
[Thrombinography: towards a globalization of coagulation tests].
1Service d'Hématologie biologique et Immuno-Hématologie, Département de Biologie clinique, CHU de Liège, Belgique. pierre.peters@chu.ulg.ac.be
Revue Medicale De Liege
|June 12, 2009
Summary
Thrombinography offers a comprehensive view of blood clotting by measuring thrombin generation kinetics. This advanced method surpasses traditional tests in detecting bleeding and clotting disorders.
Area of Science:
- Hematology
- Biochemistry
- Physiology
Context:
- Thrombin is central to hemostasis, but conventional clotting tests (PT, aPTT) primarily detect initial fibrin formation.
- These tests often miss significant thrombin generation occurring after clot detection, limiting their diagnostic sensitivity for many hemostatic disorders.
Purpose:
- To introduce thrombinography as a method for measuring thrombin generation and inactivation kinetics under standardized ex vivo conditions.
- To highlight the clinical utility of thrombinography in diagnosing and managing various hemorrhagic and thrombotic conditions.
Summary:
- Thrombinography assesses the complete thrombin generation profile, unlike traditional clotting assays.
- Reduced thrombin generation is observed in hemophilia and with anticoagulant therapy.
- Elevated thrombin activity indicates hypercoagulable states (e.g., antithrombin deficiency, Factor V Leiden) and is seen with oral contraceptive use.
- Thrombin generation is delayed yet amplified with lupus anticoagulants.
- In platelet-rich plasma, it aids in diagnosing thrombopathies, von Willebrand disease, and monitoring antiplatelet therapies.
Impact:
- Thrombinography provides a global phenotype of the thrombosis-hemostasis system, offering deeper insights than standard tests.
- It enables more accurate diagnosis and management of complex bleeding and clotting disorders.
- This method has potential for monitoring anticoagulant and antiplatelet therapies more effectively.
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