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Updated: Jun 22, 2026
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
Published on: May 24, 2012
[Construction of retroviral vector with human brain-derived neurotrophic factor gene expression and in the
Cheng Yang1, Rongzeng Yan, Hongyan Guo
1Stomatological Center, General Hospital of Chinese People's Armed Police Forces, Beijing, 100039, P.R. China.
Objective:
To construct human brain-derived neurotrophic factor retroviral vector-pLXSN (hBDNF-pLXSN), and to evaluate the bioactivity of hBDNF.
Methods:
The genome mRNA was extracted from embryonic brain tissue of a 5-month-old infant, the hBDNF gene sequence was obtained with RT-PCR technology, and hBDNF-pLXSN constructed in vitro was used to infect the fibroblasts (NIH/3T3). The expression of hBDNF was identfied by the immunohistochemistry method, and the NIH/3T3 and BDNF biological activities were determined by culture of the PC12 cells and dorsal root ganglia.
Results:
The hBDNF-pLXSN was constructed successfully by sequencing analyses. The infected NIH/3T3 showed positive expression of hBDNF. The infected NIH/3T3 could product hBDNF. Bioactivity of the products could support the PC12 cell survival and neurite growth in the primary cultures of dorsal root ganglia neurons of mice.
Conclusion:
hBDNF-pLXSN virus has the ability to infect NIH/3T3 and make it expressed and secreted hBDNF with the biological activity. It can be used to treat facial paralysis as a gene therapy.
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