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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jun 22, 2026

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

Identification of novel functional differences in monocyte subsets using proteomic and transcriptomic methods.

Changqing Zhao1, Huoming Zhang, Wing-Cheong Wong

  • 1Singapore Immunology Network, Agency for Science, Technology and Research, Biopolis, Singapore.

Journal of Proteome Research
|June 12, 2009
PubMed
Summary
This summary is machine-generated.

This study reveals distinct functions for human blood monocyte subsets (CD14+CD16- and CD14+/lowCD16+) using proteomics and transcriptomics. Novel gene expression differences highlight their unique roles in immune responses.

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Last Updated: Jun 22, 2026

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Area of Science:

  • Immunology
  • Cell Biology
  • Proteomics
  • Transcriptomics

Background:

  • Human blood monocytes comprise CD14+CD16- and CD14+/lowCD16+ subsets with altered proportions in disease.
  • While phenotypic and functional differences are known, their specific roles in health and disease remain largely undefined.

Purpose of the Study:

  • To uncover novel functional differences between monocyte subsets through global proteomics and transcriptomics.
  • To elucidate the distinct roles of CD14+CD16- and CD14+/lowCD16+ monocytes in immune regulation.

Main Methods:

  • Global proteomics and transcriptomics analysis of CD14+CD16- and CD14+/lowCD16+ monocyte subsets.
  • Identification and assessment of 613 genes with differential protein and mRNA expression (fold change >= |1.5|).
  • In silico mapping of up-regulated genes to biological functions.

Main Results:

  • Significant functional differences were identified between the two monocyte subsets at both protein and mRNA levels.
  • CD14+/lowCD16+ monocytes showed up-regulated genes in Fc receptor-mediated phagocytosis.
  • CD14+CD16- monocytes exhibited up-regulated genes related to antimicrobial functions.

Conclusions:

  • Novel functional distinctions between monocyte subsets were uncovered via integrated proteomic and transcriptomic analyses.
  • These findings provide new insights into the differential roles of monocyte subsets in innate and adaptive immunity.