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Related Experiment Video

Updated: Jun 22, 2026

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Published on: May 3, 2011

EST and EST-SSR marker resources for Iris.

Shunxue Tang1, Rebecca A Okashah, Marie-Michele Cordonnier-Pratt

  • 1Institute of Plant Breeding, Genetics, and Genomics, The University of Georgia, Athens, 30602, USA. tangs@uga.edu

BMC Plant Biology
|June 12, 2009
PubMed
Summary

Researchers developed over 400 simple sequence repeat (SSR) markers for Iris species. These markers are highly variable and useful for genotyping diverse Iris cultivars and wild ecotypes.

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Area of Science:

  • Genomics
  • Plant Science
  • Molecular Biology

Background:

  • Limited DNA resources exist for the monocot genus Iris (Iridaceae), despite its horticultural importance.
  • Developing genetic markers is crucial for understanding Iris diversity and applications.

Purpose of the Study:

  • To mine expressed sequence tags (ESTs) for simple sequence repeats (SSRs) in Iris.
  • To develop ortholog-specific EST-SSR markers for genetic mapping and genotyping in Iris.
  • To assess the cross-species utility and polymorphism of these markers.

Main Methods:

  • ESTs from I. brevicaulis and I. fulva were assembled into unigenes.
  • SSRs were identified within unigenes, and EST-SSR markers were designed.
  • Marker amplification and polymorphism were tested across various Iris species, ecotypes, and cultivars.

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Last Updated: Jun 22, 2026

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Published on: May 3, 2011

Iris Fixation via External Pentagram Suturing
05:22

Iris Fixation via External Pentagram Suturing

Published on: May 5, 2022

Main Results:

  • 526 EST-SSR markers were developed from 4,917 unigenes.
  • Nearly 400 markers (399/526) amplified alleles in I. brevicaulis and I. fulva.
  • A high percentage of markers (84%) were polymorphic between parents of mapping populations.
  • Markers showed broad amplification in wild Louisiana Iris ecotypes and varied utility in other cultivated species.
  • Significant genetic diversity was observed, with 2-18 alleles/locus and a mean heterozygosity of 0.76.

Conclusions:

  • Developed nearly 400 ortholog-specific EST-SSR markers for Iris.
  • These markers are highly polymorphic and suitable for comparative genetic mapping.
  • The markers demonstrate broad utility for genotyping applications across the Iris genus.