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Updated: Jun 22, 2026

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
Published on: September 29, 2023
Synthesis of Lewis X epitopes on plant N-glycans
Gerard J A Rouwendal1, Dion E A Florack, Thamara Hesselink
1Plant Research International B.V., Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands. gerard.rouwendal@synthon.nl
Abstract:
Glycoproteins from tobacco line xFxG1, in which expression of a hybrid beta-(1-->4)-galactosyltransferase (GalT) and a hybrid alpha-(1-->3)-fucosyltransferase IXa (FUT9a) is combined, contained an abundance of hybrid N-glycans with Lewis X (Le(X)) epitopes. A comparison with N-glycan profiles from plants expressing only the hybrid beta-(1-->4)-galactosyltransferase suggested that the fucosylation of the LacNAc residues in line xFxG1 protected galactosylated N-glycans from endogenous plant beta-galactosidase activity.
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