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First experimental identification of Ras-inhibitor binding interface using a water-soluble Ras ligand
Alessandro Palmioli1, Elena Sacco, Sherwin Abraham
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza, 2, 20126 Milano, Italy.
Bioorganic & Medicinal Chemistry Letters
|June 12, 2009
Abstract:
By combining in the same molecule Ras-interacting aromatic moieties and a sugar, we prepared a water-soluble Ras ligand that binds Ras and inhibits guanine nucleotide exchange. With this compound it was possible to determine experimentally by a (15)N-edited HSQC NMR experiment the ligand-Ras binding interface.
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