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Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
Published on: October 22, 2012
Exercise training, NADPH oxidase p22phox gene polymorphisms, and hypertension
Deborah L Feairheller1, Michael D Brown, Joon-Young Park
1Hypertension, Molecular and Applied Physiology Laboratory, Department of Kinesiology, Temple University, Philadelphia, PA 19122, USA. dlf@temple.edu
Medicine and Science in Sports and Exercise
|June 12, 2009
Summary
Aerobic exercise training significantly impacts oxidative stress biomarkers in hypertensive individuals. While CYBA gene variants influence baseline levels, exercise training is the primary driver of changes in these markers.
Area of Science:
- Cardiovascular Physiology
- Genetics
- Exercise Science
Background:
- Oxidative stress, mediated by NADPH oxidase, contributes to hypertension pathology.
- Aerobic exercise training is known to reduce NADPH oxidase activity.
- The role of CYBA gene variations in oxidative stress response to exercise in hypertensives remains understudied.
Purpose of the Study:
- To investigate the association between CYBA gene polymorphisms (C242T and A640G) and oxidative stress markers.
- To determine the impact of aerobic exercise training on these markers in Pre and Stage 1 hypertensive adults.
- To examine if CYBA genotype influences individual responses to exercise interventions.
Main Methods:
- Ninety-four sedentary hypertensive adults participated in a 6-month aerobic exercise program (70% VO2max).
- Measurements included urinary 8-iso-prostaglandin F2alpha (8-iso-PGF2alpha), urinary nitric oxide metabolites (NOx), and plasma total antioxidant capacity (TAC).
- CYBA C242T and A640G polymorphisms were genotyped and analyzed for associations with oxidative stress biomarker changes.
Main Results:
- Baseline 8-iso-PGF2alpha levels differed significantly across C242T genotypes (TT lowest, CC highest).
- Aerobic exercise training significantly increased VO2max, urinary 8-iso-PGF2alpha, and plasma TAC.
- Significant decreases in urinary NOx were observed post-training.
- No significant differences in exercise-induced changes in oxidative stress markers were found among CYBA genotype groups.
Conclusions:
- Aerobic exercise training demonstrated a greater influence on oxidative stress biomarkers than CYBA polymorphisms.
- The CYBA C242T variant was associated with baseline 8-iso-PGF2alpha levels.
- Aerobic exercise-induced responses in oxidative stress markers were not significantly modulated by the studied CYBA variants.
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