Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Environmental Drivers of Communal Roost Distribution and Size in Western Jackdaws (Coloeus monedula) Under Landscape Transformation.

Integrative zoology·2026
Same author

Large-scale interspecific associations and ecological context shape communal roosts of Western jackdaw (Coloeus monedula).

PloS one·2026
Same author

Complexity matching: adaptive strong anticipation enhances motor coordination.

Experimental brain research·2026
Same author

Questions and issues of nonventilated hospital-acquired pneumonia: an opinion document.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same author

Optometrist-guided versus self-driven subjective refraction using tunable optics: quantifying the professional's impact.

Journal of optometry·2026
Same author

American College of Chest Physicians algorithm for lung resective surgery: Real-life validation.

Pulmonology·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Superresolution optical system by common-path interferometry.

Vicente Mico, Zeev Zalevsky, Javier García

    Optics Express
    |June 12, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new method for superresolved imaging in digital holography. This approach enhances optical resolution using synthetic aperture generation and off-axis illumination, simplifying complex systems.

    More Related Videos

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

    Published on: December 1, 2016

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
    12:22

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

    Published on: August 4, 2018

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

    Published on: December 1, 2016

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
    12:22

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

    Published on: August 4, 2018

    Area of Science:

    • Optical Imaging
    • Holography
    • Superresolution Microscopy

    Background:

    • Digital holography offers advanced imaging capabilities.
    • Improving optical resolution is crucial for detailed microscopic analysis.
    • Current methods often involve system complexity.

    Purpose of the Study:

    • To present a novel, simplified approach for superresolved digital holography.
    • To enhance the optical resolution limit in imaging systems.
    • To demonstrate a practical method using common-path interferometry and off-axis illumination.

    Main Methods:

    • Synthetic aperture generation using common-path interferometry.
    • Off-axis illumination with a Vertical Cavity Surface Emitting Laser (VCSEL) array.
    • Integration of a diffraction grating with the object in the input plane.

    Main Results:

    • Achieved a doubling of optical resolution using tilted illumination.
    • Demonstrated significant improvement in optical resolution limit with a novel grating-based approach.
    • Experimental validation using commercial microscope objectives.

    Conclusions:

    • The proposed methods offer effective strategies for superresolved imaging in digital holography.
    • The novel approach significantly enhances optical resolution with minimal system complexity.
    • This technique holds promise for advanced optical imaging applications.