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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Colloidal precipitates01:09

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Enhanced optical guiding of colloidal particles using a supercontinuum light source.

P Fischer, A E Carruthers, K Volke-Sepulveda

    Optics Express
    |June 12, 2009
    PubMed
    Summary

    Supercontinuum light beams significantly extend optical guiding distances for microscopic particles, doubling the range compared to traditional laser sources. This breakthrough offers new possibilities for manipulating small objects.

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    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
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    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
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    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

    Published on: January 3, 2016

    Area of Science:

    • Optics and Photonics
    • Particle Manipulation
    • Soft Matter Physics

    Background:

    • Optical trapping and guiding rely on forces exerted by light on particles.
    • Traditional methods using monochromatic beams have limited guiding distances due to rapid focal region decay.
    • Supercontinuum sources offer broad spectral bandwidth, potentially enabling novel optical phenomena.

    Purpose of the Study:

    • To investigate the potential of supercontinuum light for enhanced optical guiding of microscopic particles.
    • To compare the guiding performance of supercontinuum beams with monochromatic Gaussian beams.
    • To determine the maximum achievable optical guiding distances using this new approach.

    Main Methods:

    • Utilizing a supercontinuum light source with broad spectral bandwidth.
    • Employing optical tweezers principles to trap and guide micron-sized particles.
    • Measuring particle displacement and stability along the beam axis.
    • Comparing results with those obtained using continuous wave (CW) and femtosecond laser sources.

    Main Results:

    • Achieved optical guiding distances of up to 3 mm for micron-sized particles.
    • Demonstrated approximately double the guiding distance compared to monochromatic laser sources of equivalent beam diameter.
    • Observed significant radial gradient force and axial radiation pressure over an elongated focal region.
    • Confirmed the enhanced spectral bandwidth of the supercontinuum source as the key factor.

    Conclusions:

    • Supercontinuum light sources provide significantly enhanced optical guiding distances for microscopic particles.
    • The elongated focal region created by the broad spectrum is responsible for the improved performance.
    • This technique shows promise for applications in manipulating colloidal particles, biological cells, and cold atom ensembles.