Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Atomic Fluorescence Spectroscopy

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...

Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

IR Spectroscopy: Molecular Vibration Overview

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Unraveling Dynamic Trap-State Modulation in Single Core-Crown CdSe/CdS Nanoplatelets.

Nano letters·2026

Same author

Collective Spectral Diffusion of Defect Luminescence in CH<sub>3</sub>NH<sub>3</sub>PbI<sub>3</sub> Halide Perovskites.

The journal of physical chemistry letters·2026

Same author

Deterministic Generation of Single B Centers in hBN by One-to-One Conversion from UV Centers.

ACS nano·2026

Same author

Resonance fluorescence and indistinguishable photons from a coherently driven B centre in hBN.

Nature communications·2026

Same author

Red and infrared colour centres in zinc oxide epitaxial nanowires.

Nanotechnology·2025

Same author

Models of light absorption enhancement in perovskite solar cells by plasmonic nanoparticles.

Exploration (Beijing, China)·2024

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference

Published on: September 5, 2019

Photon-correlation Fourier spectroscopy.

Xavier Brokmann, Moungi Bawendi, Laurent Coolen

|June 12, 2009

Summary

This summary is machine-generated.

This study presents a new method to analyze spectral fluctuations in single molecules using fluorescence intensity correlations. It achieves high spectral and temporal resolution, even with low light levels.

More Related Videos

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Related Experiment Videos

Last Updated: Jun 22, 2026

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference

Published on: September 5, 2019

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Area of Science:

Spectroscopy
Quantum Optics
Single-Molecule Biophysics

Background:

Understanding spectral fluctuations is crucial for characterizing light sources.
Standard spectroscopy methods face limitations in time resolution for faint light sources.

Purpose of the Study:

To develop a novel method for probing spectral fluctuations.
To achieve high spectral and temporal resolution in analyzing faint light sources.

Main Methods:

Utilizing Fourier spectroscopy for high spectral resolution.
Employing a continuously scanning Michelson interferometer.
Analyzing fluorescence intensity correlations of a single radiating dipole.

Main Results:

Demonstrated a method to probe spectral fluctuations over broad frequency and timescale ranges.
Achieved temporal resolution comparable to the excited state lifetime.
Enabled analysis even at very low photocounting rates.

Conclusions:

The developed method offers a new approach to investigate fast fluorescence spectral dynamics.
This technique extends beyond the time-resolution limits of standard spectroscopy.
Applicable to single molecules and other faint light sources.