Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of Soft Tissue N-Glycome Profiles in Oral Squamous Cell Carcinoma, a Pilot Study.

International journal of molecular sciences·2026
Same author

Capillary Gradient Gel Electrophoresis.

Gels (Basel, Switzerland)·2026
Same author

Preface.

Current molecular medicine·2025
Same author

IgG N-Glycan Profiles in Mothers and Infants Postpartum in the Context of Maternal Obesity and Gestational Diabetes.

International journal of molecular sciences·2025
Same author

HUPO 2024 CONFERENCE REPORT.

Current molecular medicine·2025
Same author

Improved analytical workflow towards machine learning supported N-glycomics-based biomarker discovery.

Talanta·2025
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

DNA sequencing by CE.

Barry L Karger1, András Guttman

  • 1The Barnett Institute, Northeastern University, Boston, MA, USA.

Electrophoresis
|June 12, 2009
PubMed
Summary
This summary is machine-generated.

Multicapillary electrophoresis (CE) systems were crucial for sequencing the Human Genome, paving the way for genomics and personalized medicine. This review highlights CE

More Related Videos

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
09:14

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

Related Experiment Videos

Last Updated: Jun 22, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
09:14

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

Area of Science:

  • Genomics
  • Biomedical Research
  • Molecular Biology

Background:

  • The Human Genome Project and the advent of personalized medicine have driven significant advancements in DNA sequencing technologies.
  • Early DNA sequencing methods, such as slab gel electrophoresis, were labor-intensive and limited in throughput.
  • The evolution of sequencing technologies is central to progress in the biomedical field.

Observation:

  • Automated multicapillary electrophoresis (CE) systems, utilizing fluorophore labeling and multispectral imaging, represented a major leap from earlier methods.
  • Next-generation sequencing (NGS) technologies, including cyclic-array, hybridization-based, nanopore, and single-molecule sequencing, are rapidly advancing the field.
  • CE played an indispensable role in the foundational sequencing of the Human Genome.

Findings:

  • Multicapillary electrophoresis systems were essential for the successful sequencing of the Human Genome.
  • The development of sequencing technologies has been a collaborative effort involving academia, healthcare professionals, and industry.
  • CE technologies provided a critical foundation for the subsequent rapid advancements in genomics.

Implications:

  • Understanding the historical role of CE provides context for the current pace of innovation in DNA sequencing.
  • The foundational work enabled by CE underpins the ongoing transition towards personalized medicine.
  • Continued technological evolution in DNA sequencing promises further breakthroughs in biomedical research and clinical applications.